Broad bean is a widely cultivated legume, known for its high resistance to harsh environmental conditions (including increased salinity, or prolonged drought). In the course of the study, we were able to demonstrate significant changes in the expression of proteins associated with the stabilization of inhibited replication forks under stressful conditions - a mechanism that works flawlessly even under prolonged exposure to environmental factors, directly contributing to the plant's considerable resilience. One of the technical achievements of the project was the dual detection of replication using two seemingly incompatible methods - the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and 5-iodo-2'-deoxyuridine (IdU). The success of the method allowed visual depiction of changes in replication activity during, and after, stress-inducing conditions. In addition, 2 3D modeling methods were developed using the open source software, Blender 3D. These methods can be successfully used by biologists without access to specialized (here, paid) programs. The research is conducted under the direction of Dorota Rybaczek, Ph.D., professor at the Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Lodz.
